| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lisica, Violet E (10)
|
MRTVSC |
32.19 |
32.01 |
-0.18 |
| 2 |
Barshay, Amanda (10)
|
MRHAA |
32.11 |
32.29 |
0.18 |
| 3 |
Kim, Julia Y (10)
|
MRTS |
33.12 |
32.32 |
-0.80 |
| 4 |
Frevola, Emma G (10)
|
MRTS |
33.94 |
32.34 |
-1.60 |
| 5 |
Hinman, Faith (10)
|
MRHAA |
34.13 |
33.00 |
-1.13 |
| 6 |
Goswami, Shreya (10)
|
MRTS |
35.12 |
33.11 |
-2.01 |
| 7 |
Guo, Alison A (10)
|
MRLIAC |
33.75 |
33.22 |
-0.53 |
| 8 |
Vietmeier, Alexandria M (10)
|
MRLIAC |
33.96 |
33.24 |
-0.72 |
| 9 |
Carey, Emilie C (10)
|
MRHAA |
34.03 |
33.25 |
-0.78 |
| 10 |
Kofsky, Alexandra I (10)
|
MRWISC |
34.12 |
33.65 |
-0.47 |
| 10 |
Bregman, Mollie E (10)
|
MRLIAC |
32.79 |
33.65 |
0.86 |
| 12 |
Kidhardt, Suvi M (10)
|
MRLIAC |
33.48 |
33.96 |
0.48 |
| 13 |
Darcy, Cate Q (10)
|
MRLIAC |
34.38 |
33.99 |
-0.39 |
| 14 |
Pan, Anna W (10)
|
MRLIAC |
34.63 |
34.19 |
-0.44 |
| 15 |
McGuigan, Aubrey J (10)
|
MRFA |
34.61 |
34.39 |
-0.22 |
| 16 |
Murat, Harper (10)
|
MRHAA |
35.12 |
34.66 |
-0.46 |
| 17 |
Rose-Freaney, Ansley V (10)
|
MRSSC |
33.87 |
34.79 |
0.92 |
|
Muety, Keira L (10)
|
MRLIAC |
30.46 |
30.40 |
-0.06 |